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LearnPA5-96793
antibody from Invitrogen Antibodies
Targeting: PSTPIP1
CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- PA5-96793 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSTPIP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AFERISANGH QKQVEKSQNK ARQCKDSATE AERVYRQSIA QLEKVRAEWE QEHRTTCEAF QLQEFDRLTI LRNALWVHSN QLSMQCVKDD ELYEEVRLTL EGCSIDADID SFIQAKSTGT EPPAPVPYQN YYDREVTPLT SSPGIQPSCG MIKRFSGLLH GSPKTTSLAA SAASTETLTP TPERNEGVYT AIAVQEIQGN PASPAQEYRA LYDYTAQNPD ELDLSAGDIL EVILEGEDGW WTVERNGQRG FVPGSYLEKL; Positive Samples: THP-1, mouse spleen, mouse thymus, mouse brain, rat brain, Mouse spleen, Mouse thymus, Mouse brain, Rat brain; Cellular Location: Cell projection, Cleavage furrow, Cytoplasm, cytoskeleton, lamellipodium, perinuclear region, uropodium
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSTPIP1 in extracts of various cell lines. Samples were incubated with PSTPIP1 Polyclonal antibody (Product # PA5-96793) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PSTPIP1 in paraffin-embedded mouse kidney. Samples were incubated with PSTPIP1 Polyclonal antibody (Product # PA5-96793) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PSTPIP1 in paraffin-embedded human stomach. Samples were incubated with PSTPIP1 Polyclonal antibody (Product # PA5-96793) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PSTPIP1 in paraffin-embedded mouse kidney. Samples were incubated with PSTPIP1 Polyclonal antibody (Product # PA5-96793) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PSTPIP1 in paraffin-embedded human stomach. Samples were incubated with PSTPIP1 Polyclonal antibody (Product # PA5-96793) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
