Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 634504 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#634504, RRID:AB_2565615
- Product name
- Alexa Fluor® 594 anti-Alix
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length human Alix protein
- Description
- Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 3A9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- NIT/3T3 cells were fixed with -20°C Methanol for ten minutes and then blocked with 5% FBS for 30 minutes. The cells were stained with 5 µg/ml of anti-Alix (Clone 3A9) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 40X objective.
- Conjugate
- Yellow dye