R32288

antibody from NSJ Bioreagents

Targeting: PDCD6IP AIP1, Alix, Hp95

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32288

Provider

NSJ Bioreagents

Product name

ALIX Antibody / PDCD6IP

Antibody type

Polyclonal

Description

This highly specific ALIX antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ALIX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis, 2) mouse testis, 3) human A375 and 4) human HeLa lysate wtih ALIX antibody. Expected/observed molecular weight ~96 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis, 2) mouse testis, 3) human A375 and 4) human HeLa lysate wtih ALIX antibody. Expected/observed molecular weight ~96 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human PANC-1, 4) rat liver, 5) rat RH35, 6) mouse liver and 7) mouse NIH 3T3 cell lysate with ALIX antibody. Expected/observed molecular weight ~96 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human T-47D cells with ALIX antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with ALIX antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung tissue with ALIX antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human esophagus squama tissue with ALIX antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine tissue with ALIX antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat liver tissue with ALIX antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.