Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9770 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ALIX/PDCD6IP Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for ALIX/PDCD6IP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. ALIX/PDCD6IP information: Molecular Weight: 96023 MW; Subcellular Localization: Cytoplasm, cytosol . Melanosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Secreted, exosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Galectin-3 mediates pulmonary vascular endothelial cell dynamics via TRPC1/4 under acute hypoxia.
Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.
CircRNAs in exosomes from high glucose-treated glomerular endothelial cells activate mesangial cells.
Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A.
Li Y, Chen X, Zeng X, Chen S, Yang X, Zhang L
Journal of biochemical and molecular toxicology 2020 May;34(5):e22463
Journal of biochemical and molecular toxicology 2020 May;34(5):e22463
Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.
Cao G, Meng X, Han X, Li J
Bioscience reports 2020 May 29;40(5)
Bioscience reports 2020 May 29;40(5)
CircRNAs in exosomes from high glucose-treated glomerular endothelial cells activate mesangial cells.
Ling L, Tan Z, Zhang C, Gui S, Cui Y, Hu Y, Chen L
American journal of translational research 2019;11(8):4667-4682
American journal of translational research 2019;11(8):4667-4682
Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A.
Du L, Li G, Yang Y, Yang G, Wan J, Ma Z, Hou Y
International journal of clinical and experimental pathology 2018;11(10):4879-4888
International journal of clinical and experimental pathology 2018;11(10):4879-4888
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ALIX using anti-ALIX antibody (PB9770). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse NIH3T3 whole cell lysates, Lane 7: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified polyclonal antibody (Catalog # PB9770) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALIX at approximately 100KD. The expected band size for ALIX is at 96KD.
- Additional image