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Learn610-401-B87
antibody from Rockland Immunochemicals, Inc.
Targeting: GDF15
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 610-401-B87 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#610-401-B87, RRID:AB_10703266
- Product name
- Anti-NAG-1 (RABBIT) Antibody - 610-401-B87
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (611-103-122) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
- Validation comment
- Western Blot
