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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-35111 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RASGRP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RASGRP2 in human spleen lysate. Samples were incubated with RASGRP2 polyclonal antibody (Product # PA5-35111) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RASGRP2 in K562 cell line lysates. Samples were incubated with RASGRP2 polyclonal antibody (Product # PA5-35111). Lysates: 35 µg/lane. RASGRP2 protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RASGRP2 in U-251 cells using a RASGRP2 polyclonal antibody (Product # PA5-35111) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue) and cytoplasmic actin was stained with a fluorescent phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of RASGRP2 in U-251MG cells. Samples were incubated in RASGRP2 polyclonal antibody (Product # PA5-35111) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RASGRP2 in formalin fixed and paraffin embedded human brain tissue. Samples were incubated with RASGRP2 polyclonal antibody (Product # PA5-35111) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RASGRP2 in formalin fixed and paraffin embedded human brain tissue. Samples were incubated with RASGRP2 polyclonal antibody (Product # PA5-35111) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
