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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-28865 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RASGRP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, mouse liver. Predicted reactivity: Mouse (96%), Rat (96%), Pig (97%), Rabbit (96%), Rhesus Monkey (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Calcium-RasGRP2-Rap1 signaling mediates CD38-induced migration of chronic lymphocytic leukemia cells.
Mele S, Devereux S, Pepper AG, Infante E, Ridley AJ
Blood advances 2018 Jul 10;2(13):1551-1561
Blood advances 2018 Jul 10;2(13):1551-1561
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RASGRP2 Polyclonal Antibody detects RASGRP2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. RASGRP2 Polyclonal Antibody (Product # PA5-28865) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RASGRP2 was performed in Raji whole cell extracts using 5 µg of RASGRP2 Polyclonal Antibody (Product # PA5-28865). Samples were transferred to a membrane and probed with RASGRP2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. The Rap1 GEF RasGRP2 is required for the enhanced migration of MEC1-CD38H cells. MEC1-GFP and MEC1-CD38H cells were transfected with siRNAs targeting RasGRP2 or RasGRP3. (A) Representative blots of RasGRP2 and RasGRP3 expression levels. (B) Migration of MEC1-GFP and MEC1-CD38H cells through transwell filters upon RasGRP2 and RasGRP3 depletion. The migration index was obtained by normalizing the values to the MEC1-GFP control cells. Data shown are the mean of 3 independent experiments +- SEM. * P < .05, determined by 2-tailed Student t test. (C) Representative blot (left) and quantification (right) of active Rap1-GTP levels in MEC1-GFP and MEC1-CD38H cells after RasGRP2 depletion. Graph shows the mean of 3 independent experiments +- SEM. * P < .05; ** P < .01 determined by 2-tailed Student t test.
