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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP53590PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53590PU-N, RRID:AB_11145035
- Product name
- anti RASGRP2 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of Human RASGRP2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
Submitted references Identification of the gene regulatory region in human rasgrp2 gene in vascular endothelial cells.
Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1.
Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling.
Guanine nucleotide exchange factors CalDAG-GEFI and CalDAG-GEFII are colocalized in striatal projection neurons.
A CalDAG-GEFI/Rap1/B-Raf cassette couples M(1) muscarinic acetylcholine receptors to the activation of ERK1/2.
Nagamine K, Matsuda A, Hori T
Biological & pharmaceutical bulletin 2010;33(7):1138-42
Biological & pharmaceutical bulletin 2010;33(7):1138-42
Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1.
Katagiri K, Shimonaka M, Kinashi T
The Journal of biological chemistry 2004 Mar 19;279(12):11875-81
The Journal of biological chemistry 2004 Mar 19;279(12):11875-81
Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling.
Eto K, Murphy R, Kerrigan SW, Bertoni A, Stuhlmann H, Nakano T, Leavitt AD, Shattil SJ
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12819-24
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12819-24
Guanine nucleotide exchange factors CalDAG-GEFI and CalDAG-GEFII are colocalized in striatal projection neurons.
Toki S, Kawasaki H, Tashiro N, Housman DE, Graybiel AM
The Journal of comparative neurology 2001 Sep 3;437(4):398-407
The Journal of comparative neurology 2001 Sep 3;437(4):398-407
A CalDAG-GEFI/Rap1/B-Raf cassette couples M(1) muscarinic acetylcholine receptors to the activation of ERK1/2.
Guo FF, Kumahara E, Saffen D
The Journal of biological chemistry 2001 Jul 6;276(27):25568-81
The Journal of biological chemistry 2001 Jul 6;276(27):25568-81
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of RASGRP2 Antibody (N-term) Cat.-No AP53590PU-NÂ in K562 cell line lysates (35ug/lane). This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term) Cat.-No AP53590PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human brain tissue stained with RASGRP2 Antibody (N-term) Cat.-No AP53590PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
