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- References [4]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN954463 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2) (N-Term), (AA 14-42) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of Human RASGRP2. Genename: RASGRP2
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 14-42
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification of the gene regulatory region in human rasgrp2 gene in vascular endothelial cells.
Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1.
Guanine nucleotide exchange factors CalDAG-GEFI and CalDAG-GEFII are colocalized in striatal projection neurons.
A CalDAG-GEFI/Rap1/B-Raf cassette couples M(1) muscarinic acetylcholine receptors to the activation of ERK1/2.
Nagamine K, Matsuda A, Hori T
Biological & pharmaceutical bulletin 2010;33(7):1138-42
Biological & pharmaceutical bulletin 2010;33(7):1138-42
Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1.
Katagiri K, Shimonaka M, Kinashi T
The Journal of biological chemistry 2004 Mar 19;279(12):11875-81
The Journal of biological chemistry 2004 Mar 19;279(12):11875-81
Guanine nucleotide exchange factors CalDAG-GEFI and CalDAG-GEFII are colocalized in striatal projection neurons.
Toki S, Kawasaki H, Tashiro N, Housman DE, Graybiel AM
The Journal of comparative neurology 2001 Sep 3;437(4):398-407
The Journal of comparative neurology 2001 Sep 3;437(4):398-407
A CalDAG-GEFI/Rap1/B-Raf cassette couples M(1) muscarinic acetylcholine receptors to the activation of ERK1/2.
Guo FF, Kumahara E, Saffen D
The Journal of biological chemistry 2001 Jul 6;276(27):25568-81
The Journal of biological chemistry 2001 Jul 6;276(27):25568-81
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of RASGRP2 Antibody (N-term) Cat.-No AP53590PU-N in K562 cell line lysates (35ug/lane). This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term) Cat.-No AP53590PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human brain tissue stained with RASGRP2 Antibody (N-term) Cat.-No AP53590PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
