MA1-40230

antibody from Invitrogen Antibodies

Targeting: COL18A1 KNO, KNO1, KS

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-40230

Provider

Invitrogen Antibodies

Product name

Endostatin Monoclonal Antibody (1837-46)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-40230 detects Endostatin from human samples. MA1-40230 has been successfully used in ELISA, flow cytometry, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot applications. For staining of paraffin sections antigen retrieval (microwave irradiation in citrate buffer) is advised. The MA1-40230 immunogen is human endostatin.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1837-46

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C

References

Submitted references

Optimized Attenuated Salmonella Typhimurium Suppressed Tumor Growth and Improved Survival in Mice.

Liang K, Zhang R, Luo H, Zhang J, Tian Z, Zhang X, Zhang Y, Ali MK, Kong Q
Frontiers in microbiology 2021;12:774490

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Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.

Yoo J, Jung JH, Lee MA, Seo KJ, Shim BY, Kim SH, Cho DG, Ahn MI, Kim CH, Cho KD, Kang SJ, Kim HK
Journal of Korean medical science 2007 Apr;22(2):318-25

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 The expression of endostatin and/or TRAIL by optimized Salmonella and their in vitro cell-killing activities. (A,B) The expression of endostatin (ES, RGD4C-ES, RGD10-ES, and PSMA-ES; 25.2, 28.1, 28.4, and 52.6 kDa) and TRAIL (34.3 kDa) by engineered Salmonella strain D2-asd was detected by western blotting, respectively. The samples tested were prepared from bacterial lysates. (C) Recombinant Salmonella co-expressing endostatin and TRAIL were also constructed and verified in the same way. (D-F) The toxicity of recombinant Salmonella bacteria expressing endostatin and/or TRAIL to HUVECs and cancer cells at the MOI of 1:100 or 1:10 were tested by the CCK-8 assay. Besides, the filtered medium supernatant of bacterial culture containing secreted anti-tumor molecules was also added to cells for testing. The cell viability shown (%) was relative to that of untreated cells, followed by comparison among groups by two-way ANOVA analysis. The cell viability shown (%) was relative to that of untreated cells. Difference significance between ST/3342 and other groups was analyzed through two-way ANOVA analysis followed by Tukey's multiple comparisons test (* p < 0.05; ** p < 0.01; and *** p < 0.001).