ABIN2508019

antibody from antibodies-online

Targeting: COL18A1 KNO, KNO1, KS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2508019

Provider

antibodies-online

Product name

anti-Collagen, Type XVIII, alpha 1 (COL18A1) antibody

Antibody type

Polyclonal

Antigen

Other

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEndostatin (human Endostatin). Anti-Human Endostatin specific antibody was purified by affinity chromatography employing immobilized hEndostatin matrix.

Reactivity

Human

Host

Rabbit

Vial size

100 μg

Storage

-20°C

Western blot

Supportive validation

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antibodies-online (provider)

Main image

Experimental details

Western blot analysis of recombinant human GDNF (hGDNF): Lanes 1, 4:  50 ng hGDNF (G-240). Lanes 2, 5:  10 ng hGDNF. Lanes 3, 4 ng hGDNF. Lanes 1-3: Anti-GDNF antibody (ABIN2511166), (1:1000). Lanes 4-5: Anti-GDNF antibody preincubated with the control peptide antigen. Expression of GDNF in rat dorsal root ganglion (DRG). Immunohistochemical staining of rat DRG longitudinal frozen section using Anti-GDNF antibody (ABIN2511166), (1:50), followed by Alexa 555-conjugated goat anti-rabbit antibody (red staining). Hoechst 33342 (blue) was used as a counterstain. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative. Expression of GDNF in rat hippocampus Immunohistochemical staining of rat hippocampus frozen sections with Anti-GDNF antibody (ABIN2511166), (1:100), (red). A. GDNF staining is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal arrows) and in some interneurons (vertical arrow). B. Calbindin D28k (green) appears in a subset of cells in the pyramidal layer. C. Merge of GDNF and calbindin demonstrates some co-localization in neurons. DAPI is used as the counterstain (blue). Expression of GDNF in rat C6 glioma cells Immunocytochemical staining of GDNF in rat C6 glioma cells. A. Paraformaldehyde-fixed and permeabilized rat C6 glioma cells were stained with Anti-GDNF antibody (ABIN2511166), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as in (A).

Immunocytochemistry

Supportive validation

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antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence