MA5-26402

antibody from Invitrogen Antibodies

Targeting: MYL7 MYL2A, MYLC2A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-26402

Provider

Invitrogen Antibodies

Product name

MYL7 Monoclonal Antibody (OTI3A8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3A8

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MYL7 Monoclonal Antibody (OTI3A8) (Product # MA5-26402) and a 19 kDa band corresponding to MYL7 was observed in iPSC differentiated to cardiomyocytes when compared to iPSC; mouse and rat heart, rat pup heart in comparison to mouse, rat brain and skeletal muscle. Whole cell extracts (30 µg lysate) of iPSC (Lane 1), iPSC to cardiomyocytes (4 days) (Lane 2), iPSC differentiated to cardiomyocytes (8 days) (Lane 3), Mouse Heart (Lane 4), Rat Heart (Lane 5), Mouse Pup Heart (Lane 6), Rat Pup Heart (Lane 7), Mouse Brain (Lane 8), Rat Brain (lane 9), Mouse Skeletal Muscle (Lane 10), Rat Skeletal Muscle (Lane 11) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). An uncharacterized band at ~40 kDa was observed in the tissues tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MYL7 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MYL7 (Product # MA5-26402) monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a MYL7 monoclonal antibody (Product # MA5-26402).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a MYL7 monoclonal antibody (Product # MA5-26402).