PA5-30789

antibody from Invitrogen Antibodies

Targeting: MYL7 MYL2A, MYLC2A

Antibody data

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Validation data

Product number: PA5-30789 - Provider product page
Provider: Invitrogen Antibodies
Product name: MYL7 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A431, HeLa, mouse heart, rat heart. Predicted reactivity: Mouse (95%), Rat (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 3.11 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MYL7 protein structure - PA5-30789 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MYL7 using 30 µg of A) A431 and B) HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MYL7 polyclonal antibody (Product # PA5-30789) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MYL7 using 50 µg of mouse heart lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MYL7 polyclonal antibody (Product # PA5-30789) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Brain (Lane 3) and Rat Brain (Lane 4). The blot was probed with Anti-MYL7 Polyclonal Antibody (Product # PA5-30769, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 19kDa band corresponding to MYL7 was observed in Heart tissues except Brain tissues which are reported to be negative.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of MYL7 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a MYL7 Polyclonal Antibody (Product # PA5-30789) at a dilution of 1:150,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using MYL7 Polyclonal Antibody (Product # PA5-30789). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MYL7 Polyclonal Antibody (Product # PA5-30789) diluted at 1:100,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using MYL7 Polyclonal Antibody (Product # PA5-30789). Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MYL7 Polyclonal Antibody (Product # PA5-30789) diluted at 1:100,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MYL7 Polyclonal Antibody detects MYL7 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MYL7 Polyclonal Antibody (Product # PA5-30789) diluted by 1:1,000.