MA1-16739

antibody from Invitrogen Antibodies

Targeting: BEST1 BEST, BMD, RP50, VMD2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16739

Provider

Invitrogen Antibodies

Product name

Bestrophin 1 Monoclonal Antibody (E6-6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody does not react with rat samples. Suggested positive control: Pig RPE (retinal Pigment Epithelium) whole cell extract.

Reactivity

Human, Mouse, Canine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

E6-6

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Xeno-free cryopreservation of adherent retinal pigmented epithelium yields viable and functional cells in vitro and in vivo.

Pennington BO, Bailey JK, Faynus MA, Hinman C, Hee MN, Ritts R, Nadar V, Zhu D, Mitra D, Martinez-Camarillo JC, Lin TC, Thomas BB, Hinton DR, Humayun MS, Lebkowski J, Johnson LV, Clegg DO
Scientific reports 2021 Mar 18;11(1):6286

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A defined subset of clonal retinal stem cell spheres is biased to RPE differentiation.

Baakdhah TW, Coles B, van der Kooy D
iScience 2021 Jun 25;24(6):102574

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of Bestrophin (68 kDa) from human RPE cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bestrophin 1 in human RPE cell lysate. Sample was incubated in Bestrophin 1 monoclonal antibody (Product # MA1-16739).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Cryopreserved REPS retain high viability, cellular identity, and secretory function post-thaw. ( a ) Schematic of overall research design indicates the characterization assays conducted on the designated day post-seeding (DPS) or day post-thaw (DPT). Non-cryopreserved REPS were maintained in culture to provide an age-matched control for each day of sample collection. ( b ) Cryopreserved REPS exhibit 95 +- 2.3% viability as measured by propidium iodide exclusion at 1 DPT (n = 26); viability of non-cryopreserved control REPS (n = 7) averaged to 97 +- 0.89% (mean +- SD., * P < 0.05, unpaired two-tailed t-test). ( c ) REPS exhibit epithelial, cuboidal morphology prior to cryopreservation (Pre-Cryo), immediately post-thaw (PT), and 1 DPT. (Phase contrast, scale bar, 100 um.) ( d ) Amount of secreted PEDF by non-cryopreserved control REPS (black triangles) and cryopreserved/thawed REPS (gray circles) as measured by ELISA. Thawed REPS exhibited a significant increase in secreted PEDF at 7 DPT compared to Pre-Cryopreservation, and also at 21 DPT compared to 7 DPT (*** P < 0.001). A significant difference was detected between thawed REPS and age-matched non-cryopreserved controls at 7 DPT (** P < 0.01) and at 21 DPT (* P < 0.05). (Pre-Cryo, n = 93; Non-Cryo, n = 10; Cryo, n = 33. Each data point represents the mean of two technical replicates. Horizontal red bar indicates mean of the biological replicates. Independent samples Kruskal-Wallis test with pairwise comparisons.) (