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Learn100-401-B14
antibody from Rockland Immunochemicals, Inc.
Targeting: CES1
CEH, CES1A1, CES1A2, CES2, HMSE, HMSE1, SES1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 100-401-B14 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-B14, RRID:AB_2276009
- Product name
- Anti-Human Liver Carboxylesterase-1 (RABBIT) Antibody - 100-401-B14
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's anti-hCE1 shows detection of hCE1 (arrowhead) transfected into U373 MG (glioblastoma) whole cell lysate (lane 2) as a 66 kDa band. Purified hCE1 (lane 4) is similarly detected. The higher molecular weight band in lane 4 may represent a trimeric form of hCE1. No reactivity is seen in the untransfected control (lane 1). No cross reactivity occurs against the human intestinal form of CES1 (lane 3. The primary antibody was used at a 1:2,500 dilution to detect purified hCE1. Personal communication, Philip Potter and Jason Hatfield, St. Jude Childrens Research Hospital, Memphis, TN.
- Validation comment
- Western Blot
