Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C18770 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C18770, RRID:AB_2076174
- Product name
- ADGRE5 / CD97 Antibody LS-C18770
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at 4°C prior to opening. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.