AF3734

antibody from R&D Systems

Targeting: ADGRE5 CD97, TM7LN1

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3734

Provider

R&D Systems

Product name

Mouse CD97 isoform 1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse CD97 isoform 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD97 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9Z0M6


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Shear stress-dependent downregulation of the adhesion-G protein-coupled receptor CD97 on circulating leukocytes upon contact with its ligand CD55.

Karpus ON, Veninga H, Hoek RM, Flierman D, van Buul JD, Vandenakker CC, vanBavel E, Medof ME, van Lier RA, Reedquist KA, Hamann J
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3740-8

---

Analysis of CD97 expression and manipulation: antibody treatment but not gene targeting curtails granulocyte migration.

Veninga H, Becker S, Hoek RM, Wobus M, Wandel E, van der Kaa J, van der Valk M, de Vos AF, Haase H, Owens B, van der Poll T, van Lier RA, Verbeek JS, Aust G, Hamann J
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6574-83

---

Analysis of CD97 expression and manipulation: antibody treatment but not gene targeting curtails granulocyte migration.

Veninga H, Becker S, Hoek RM, Wobus M, Wandel E, van der Kaa J, van der Valk M, de Vos AF, Haase H, Owens B, van der Poll T, van Lier RA, Verbeek JS, Aust G, Hamann J
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6574-83

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD97 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD97 isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3734, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).