LS-C817880

antibody from LSBio

Targeting: ADGRE5 CD97, TM7LN1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C817880

Provider

LSBio

Product name

ADGRE5 / CD97 Antibody (Preservative Free) LS-C817880

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg SW480 Whole Cell Lysate. 0.5 uL anti-CD97 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg SW480 Whole Cell Lysate. 0.5 uL anti-CD97 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa.