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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10186 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10186, RRID:AB_1671694
- Product name
- Cd97 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant Cd97.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot after reconstitution to avoid repeated freezing and thawing.
Submitted references CD97, an adhesion receptor on inflammatory cells, stimulates angiogenesis through binding integrin counterreceptors on endothelial cells.
Proteolytic cleavage of the EMR2 receptor requires both the extracellular stalk and the GPS motif.
CD97, but not its closely related EGF-TM7 family member EMR2, is expressed on gastric, pancreatic, and esophageal carcinomas.
Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler-Stevenson WG, Kelly K
Blood 2005 Apr 1;105(7):2836-44
Blood 2005 Apr 1;105(7):2836-44
Proteolytic cleavage of the EMR2 receptor requires both the extracellular stalk and the GPS motif.
Chang GW, Stacey M, Kwakkenbos MJ, Hamann J, Gordon S, Lin HH
FEBS letters 2003 Jul 17;547(1-3):145-50
FEBS letters 2003 Jul 17;547(1-3):145-50
CD97, but not its closely related EGF-TM7 family member EMR2, is expressed on gastric, pancreatic, and esophageal carcinomas.
Aust G, Steinert M, Schütz A, Boltze C, Wahlbuhl M, Hamann J, Wobus M
American journal of clinical pathology 2002 Nov;118(5):699-707
American journal of clinical pathology 2002 Nov;118(5):699-707
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Cd97 polyclonal antibody (Cat # PAB10186) shows detection of bands corresponding to free Fc-Cd97- (5EGF) (lower arrowhead) and Fc-Cd97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells.The left lane contains lysate from cells transfected with control DNA.The right lane contains lysate from COS cells expressing Fc-Cd97- (5EGF).No staining was noted from bone marrow lysates taken from Cd97 knockout mice.The identity of the band at ~65 KDa appearing in all lanes is not known.The formation of the Cd97 complex is currently under investigation.Approximately 10 uL of lysate was used in each lane.A 1:1,000 dilution of the primary antibody was used.The image was processed using a 10-sec exposure.Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
