200-401-A57

antibody from Rockland Immunochemicals, Inc.

Targeting: TMBIM1 LFG3, PP1201, RECS1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-401-A57

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-401-A57, RRID:AB_2204048

Product name

Anti-TMBIM1 (RABBIT) Antibody - 200-401-A57

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

A shear stress responsive gene product PP1201 protects against Fas-mediated apoptosis by reducing Fas expression on the cell surface.

Shukla S, Fujita K, Xiao Q, Liao Z, Garfield S, Srinivasula SM
Apoptosis : an international journal on programmed cell death 2011 Feb;16(2):162-73

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's protein A purified anti-TMBIM1 antibody shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize overexpressed TMBIM1 but not endogenous protein. The membrane was probed with the primary antibody at a 1:1,000 dilution at 4° C, overnight. Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.

Validation comment

Western Blot