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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- MA5-34664 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS26A Recombinant Rabbit Monoclonal Antibody (JB93-41)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Rat kidney tissue , A431, human tonsil tissue, rat kidney tissue, mouse testis tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB93-41
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Vps26a was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VPS26A Recombinant Rabbit Monoclonal Antibody (JB93-41) (Product # MA5-34664) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing endosomal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of VPS26 in paraffin-embedded mouse testis tissue. Samples were incubated with VPS26 monoclonal antibody (Product # MA5-34664), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of VPS26 in paraffin-embedded rat kidney tissue. Samples were incubated with VPS26 monoclonal antibody (Product # MA5-34664), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of VPS26 in paraffin-embedded human tonsil tissue. Samples were incubated with VPS26 monoclonal antibody (Product # MA5-34664), and followed by hematoxylin.
