14859-1-AP

antibody from Proteintech Group

Targeting: PSMB8 beta5i, D6S216E, LMP7, PSMB5i, RING10

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

14859-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14859-1-AP, RRID:AB_2268923

Product name

PSMB8 antibody

Antibody type

Polyclonal

Description

PSMB8 antibody (Cat. #14859-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Degradation of IL-4Ralpha by Immunoproteasome: implication in airway type 2 inflammation and hyperresponsiveness.

Schaunaman N, Cervantes D, Ferrington DA, Chu HW
Frontiers in immunology 2025;16:1501898

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Novel insights into the molecular mechanisms of sepsis-associated acute kidney injury: an integrative study of GBP2, PSMB8, PSMB9 genes and immune microenvironment characteristics.

Ye H, Zhang X, Li P, Wang M, Liu R, Yang D
BMC nephrology 2025 Mar 29;26(1):160

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Proteasome dysfunction in T cells causes immunodeficiency via cell cycle disruption and apoptosis.

Shinebaatar E, Morimoto J, Koga R, Nguyen TN, Sasaki Y, Yonemura S, Kosako H, Yasutomo K
International immunology 2025 Jul 22;37(8):493-505

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Antitumor efficacy of intermittent low-dose erlotinib plus sulindac via MHC upregulation and remodeling of the immune cell niche.

Tripathi C, Tovar Perez JE, Kapoor S, Muhsin A, Dashwood WM, Demirhan Y, Demirhan M, Shapiro A, Mohammed A, Sei S, Thompson J, Zaheer M, Sinha KM, Brown PH, Savage MI, Vilar E, Rajendran P, Dashwood RH
International journal of cancer 2025 Jul 15;157(2):355-370

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ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation.

Chen SW, Chu Y, Chu CH, Pham XT, Ng HP, Guo CL, Cheng PL
Cell reports 2025 Jan 28;44(1):115161

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CGRPβ suppresses the pathogenesis of ulcerative colitis via the immunoproteasome.

Shibao T, Hase H, Mizokami K, Usui A, Kitae K, Ueda Y, Jingushi K, Tsujikawa K
Scientific reports 2025 Feb 28;15(1):7224

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Immunoproteasome subunits are novel signatures for predicting efficacy of immunotherapy in muscle invasive bladder cancer.

Wang X, You H, Zhang T, Li Y, Chen X, Basler M, Jiang Q, Chen H, Liu N, Yuan F, Li J
Journal of translational medicine 2025 Feb 26;23(1):228

---

Neuronal hyperactivity becomes mTORC1 independent due to transcriptional changes in tuberous sclerosis complex disease models.

Afshar-Saber W, Ruiz JF, Gisser I, Yang Z, Mehendale L, Teaney NA, Hobson R, Glass MR, Pham TT, Bainbridge E, Sahin M, Winden KD
Cell reports 2025 Dec 23;44(12):116664

---

Isoliquiritigenin alleviates experimental autoimmune encephalomyelitis by modulating inflammatory and neuroprotective reactive astrocytes.

Zhang YL, Qu Y, Song HH, Cheng G, Lu F, Cui TT, Gong Y, Ding XL, Yang Y, Zhang Q, Yang LT, Yan YP
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Sep;178:117188

---

Gemcitabine Modulates HLA-I Regulation to Improve Tumor Antigen Presentation by Pancreatic Cancer Cells.

Larson AC, Knoche SM, Brumfield GL, Doty KR, Gephart BD, Moore-Saufley PR, Solheim JC
International journal of molecular sciences 2024 Mar 11;25(6)

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Exploring the crosstalk molecular mechanisms between IgA nephropathy and Sjögren's syndrome based on comprehensive bioinformatics and immunohistochemical analyses.

He P, Wei L, Zhang R, Zhao J, Zhang Y, Huang L, Bai X, Ning X, Sun S
Clinical and experimental medicine 2024 Aug 13;24(1):188

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A prognostic mathematical model based on tumor microenvironment-related genes expression for breast cancer patients.

Chen H, Wang S, Zhang Y, Gao X, Guan Y, Wu N, Wang X, Zhou T, Zhang Y, Cui D, Wang M, Zhang D, Wang J
Frontiers in oncology 2023;13:1209707

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β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium.

Hua F, Zhu H, Yu W, Zheng Q, Zhang L, Liang W, Lin Y, Xiao F, Yi P, Xiong Y, Dong Y, Li H, Fang L, Liu H, Ying J, Wang X
Journal of neuroinflammation 2023 May 11;20(1):113

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Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4(+) T cells in chronic liver injury.

Zhang Y, Yang X, Bi T, Wu X, Wang L, Ren Y, Ou Y, Xie C, Li K, Ran H, Wang J, Zhao F, Shui P, Qing J
International immunopharmacology 2022 Jun;107:108639

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Airway epithelial immunoproteasome subunit LMP7 protects against rhinovirus infection.

Dimasuay KG, Schaunaman N, Berg B, Cervantes D, Kruger E, Heppner FL, Ferrington DA, Chu HW
Scientific reports 2022 Aug 25;12(1):14507

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Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction.

Sasaki Y, Arimochi H, Otsuka K, Kondo H, Tsukumo SI, Yasutomo K
JCI insight 2022 Apr 8;7(7)

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PITHD1 is a proteasome-interacting protein essential for male fertilization.

Kondo H, Matsumura T, Kaneko M, Inoue K, Kosako H, Ikawa M, Takahama Y, Ohigashi I
The Journal of biological chemistry 2020 Feb 7;295(6):1658-1672

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miR-451a inhibits cancer growth, epithelial-mesenchymal transition and induces apoptosis in papillary thyroid cancer by targeting PSMB8.

Fan X, Zhao Y
Journal of cellular and molecular medicine 2019 Dec;23(12):8067-8075

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP(PSMB8 antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PSMB8 antibody 14859-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lymphoma using 14859-1-AP(PSMB8 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lymphoma using 14859-1-AP(PSMB8 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue