AF7710

antibody from Novus Biologicals

Targeting: PSMB8 beta5i, D6S216E, LMP7, PSMB5i, RING10

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7710

Provider

Novus Biologicals

Product name

Sheep Polyclonal LMP7/PSMB8 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human LMP7/PSMB8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PSMB5 is observed.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human LMP7/PSMB8 by Western Blot. Western blot shows lysates of human dendritic cells, Jurkat human acute T cell leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, and HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LMP7/PSMB8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7710) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMP7/PSMB8 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.