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LearnH00005696-M01
antibody from Novus Biologicals
Targeting: PSMB8
beta5i, D6S216E, LMP7, PSMB5i, RING10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00005696-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005696-M01, RRID:AB_547405
- Product name
- Mouse Monoclonal LMP7/PSMB8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PSMB8 - proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional protease 7)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Microsatellite instability derived JAK1 frameshift mutations are associated with tumor immune evasion in endometrioid endometrial cancer.
Endoplasmic reticulum stress activates autophagy but not the proteasome in neuronal cells: implications for Alzheimer's disease.
Down-regulation of proteasomal subunit MB1 is an independent predictor of improved survival in ovarian cancer.
Stelloo E, Versluis MA, Nijman HW, de Bruyn M, Plat A, Osse EM, van Dijk RH, Nout RA, Creutzberg CL, de Bock GH, Smit VT, Bosse T, Hollema H
Oncotarget 2016 Jun 28;7(26):39885-39893
Oncotarget 2016 Jun 28;7(26):39885-39893
Endoplasmic reticulum stress activates autophagy but not the proteasome in neuronal cells: implications for Alzheimer's disease.
Nijholt DA, de Graaf TR, van Haastert ES, Oliveira AO, Berkers CR, Zwart R, Ovaa H, Baas F, Hoozemans JJ, Scheper W
Cell death and differentiation 2011 Jun;18(6):1071-81
Cell death and differentiation 2011 Jun;18(6):1071-81
Down-regulation of proteasomal subunit MB1 is an independent predictor of improved survival in ovarian cancer.
Leffers N, Gooden MJ, Mokhova AA, Kast WM, Boezen HM, Ten Hoor KA, Hollema H, Daemen T, van der Zee AG, Nijman HW
Gynecologic oncology 2009 May;113(2):256-63
Gynecologic oncology 2009 May;113(2):256-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of PSMB8 expression in transfected 293T cell line by PSMB8 monoclonal antibody (M01), clone 1B3.Lane 1: PSMB8 transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Detection limit for recombinant GST tagged PSMB8 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of monoclonal antibody to PSMB8 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of PSMB8 transfected lysate using anti-PSMB8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMB8 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (1B3) [H00005696-M01] - Analysis of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
