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LearnPA1-972-A488
antibody from Invitrogen Antibodies
Targeting: PSMB8
beta5i, D6S216E, LMP7, PSMB5i, RING10
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- PA1-972-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB8 Polyclonal Antibody, Alexa Fluor™ 488
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1972A488 detects proteasome 20S LMP7 from human cells. PA1972A488 immunizing peptide corresponds to amino acid residues 259-274 from human proteasome 20S LMP7 protein. This sequence is completely conserved between human, mouse, rat and sheep. PA1972A488 immunizing peptide (Cat. # PEP-132) is available for use in neutralization and control experiments.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Proteasome 20S LMP7 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Proteasome 20S LMP7 Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-972-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
