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LearnMA5-34676
antibody from Invitrogen Antibodies
Targeting: PSMB8
beta5i, D6S216E, LMP7, PSMB5i, RING10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- MA5-34676 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB8 Recombinant Rabbit Monoclonal Antibody (JB54-32)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U937, Rat epididymis tissue, human liver tissue, human kidney tissue, mouse small intestine tissue, A431, HUVEC, Daudi.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB54-32
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Proteasome 20S LMP7 in U937 (1) and A431 (2) cell lysate. Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Proteasome 20S LMP7 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Proteasome 20S LMP7 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Proteasome 20S LMP7 in paraffin-embedded rat epididymis tissue. Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Proteasome 20S LMP7 in paraffin-embedded rat epididymis tissue. Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Proteasome 20S LMP7 in paraffin-embedded mouse small intestine tissue. Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Proteasome 20S LMP7 in paraffin-embedded human liver tissue. Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Proteasome 20S LMP7 in paraffin-embedded human kidney tissue. Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Proteasome 20S LMP7 in Daudi cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Proteasome 20S LMP7 monoclonal antibody (Product # MA5-34676) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
