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LearnMA5-15890
antibody from Invitrogen Antibodies
Targeting: PSMB8
beta5i, D6S216E, LMP7, PSMB5i, RING10
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA5-15890 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB8 Monoclonal Antibody (1A5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15890 targets PSMB8 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and rat samples. The MA5-15890 immunogen is purified recombinant fragment of human PSMB8 expressed in E. Coli. . MA5-15890 detects PSMB8 which has a predicted molecular weight of approximately 30kDa.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A5
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The impact of regional astrocyte interferon-γ signaling during chronic autoimmunity: a novel role for the immunoproteasome.
Smith BC, Sinyuk M, Jenkins JE 3rd, Psenicka MW, Williams JL
Journal of neuroinflammation 2020 Jun 12;17(1):184
Journal of neuroinflammation 2020 Jun 12;17(1):184
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using PSMB8 monoclonal antibody (Product # MA5-15890) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using PSMB8 monoclonal antibody (Product # MA5-15890) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 monoclonal antibody (Product # MA5-15890) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 monoclonal antibody (Product # MA5-15890) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Regional astrocyte oxidative stress and iP expression in chronic MS WMLs. a and b RNA was extracted from the cortical WM of control and MS ( n = 7-12) patients and analyzed via microarray and the relative expression of ( a ) PSMB7 and ( b ) PSMB8 were assessed as described []. c and d MS demyelinating lesions (black outline) and NAWM (white square) were identified by PLP labeling of the ( c ) brainstem and ( d ) spinal cord in MS patients. e GFAP + astrocytes (red) were co-labeled with either LMP7 or PRDX6 (green) in NAWM or in WML of the brainstem or spinal cord. Scale bar: 10 muM. * P < 0.05, ** P < 0.01. Ctrl WM control white matter, NAWM normal-appearing white matter, WML white matter lesion
