MA5-15890

antibody from Invitrogen Antibodies

Targeting: PSMB8 beta5i, D6S216E, LMP7, PSMB5i, RING10

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15890

Provider

Invitrogen Antibodies

Product name

PSMB8 Monoclonal Antibody (1A5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15890 targets PSMB8 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and rat samples. The MA5-15890 immunogen is purified recombinant fragment of human PSMB8 expressed in E. Coli. . MA5-15890 detects PSMB8 which has a predicted molecular weight of approximately 30kDa.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1A5

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The impact of regional astrocyte interferon-γ signaling during chronic autoimmunity: a novel role for the immunoproteasome.

Smith BC, Sinyuk M, Jenkins JE 3rd, Psenicka MW, Williams JL
Journal of neuroinflammation 2020 Jun 12;17(1):184

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSMB8 using a PSMB8 monoclonal antibody (Product # MA5-15890) against a human PSMB8 (AA: 1-272) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSMB8 using a PSMB8 monoclonal antibody (Product # MA5-15890) against a human PSMB8 (AA: 1-272) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSMB8 using PSMB8 monoclonal antibody (Product # MA5-15890) in HeLa (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using PSMB8 monoclonal antibody (Product # MA5-15890) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using PSMB8 monoclonal antibody (Product # MA5-15890) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 monoclonal antibody (Product # MA5-15890) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 monoclonal antibody (Product # MA5-15890) followed with DAB staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Regional astrocyte oxidative stress and iP expression in chronic MS WMLs. a and b RNA was extracted from the cortical WM of control and MS ( n = 7-12) patients and analyzed via microarray and the relative expression of ( a ) PSMB7 and ( b ) PSMB8 were assessed as described []. c and d MS demyelinating lesions (black outline) and NAWM (white square) were identified by PLP labeling of the ( c ) brainstem and ( d ) spinal cord in MS patients. e GFAP + astrocytes (red) were co-labeled with either LMP7 or PRDX6 (green) in NAWM or in WML of the brainstem or spinal cord. Scale bar: 10 muM. * P < 0.05, ** P < 0.01. Ctrl WM control white matter, NAWM normal-appearing white matter, WML white matter lesion