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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-27663 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCN10A Monoclonal Antibody (S134)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-27663 was sufficient for detection of Nav1.8 in 10 µg of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa. No cross reactivity against other Nav channels.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S134
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of SCN10A in human HaCaT cells. Sample was fixed with 100% cold methanol (10 minutes at -20°C), incubated with SCN10A monoclonal antibody (Product # MA5-27663) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) secondary antibody at a dilution of 1:50 (1 hour at RT).
