GTX24080

antibody from GeneTex

Targeting: USP7 HAUSP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

GTX24080

Provider

GeneTex

Proper citation

GeneTex Cat#GTX24080, RRID:AB_373113

Product name

USP7 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex affinity purified anti-HAUSP antibody (GTX24080) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex affinity purified anti-HAUSP antibody (GTX24080) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.