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- Product number
- GTX631108 - Provider product page
- Provider
- GeneTex
- Product name
- USP7 antibody [GT481]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Human Herpesvirus 8 Interferon Regulatory Factors 1 and 3 Mediate Replication and Latency Activities via Interactions with USP7 Deubiquitinase.
Xiang Q, Ju H, Li Q, Mei SC, Chen D, Choi YB, Nicholas J
Journal of virology 2018 Apr 1;92(7)
Journal of virology 2018 Apr 1;92(7)
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- USP7 antibody [GT481] detects USP7 protein by western blot analysis.A. 30 £gg 293T whole cell extractB. 30 £gg A431 whole cell extractC. 30 £gg HeLa whole cell extractD. 30 £gg HepG2 whole cell extract5 % SDS-PAGEUSP7 antibody [GT481] (GTX631108) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- USP7 antibody [GT481] detects USP7 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP7 protein stained by USP7 antibody [GT481] (GTX631108) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 £gg of USP7 antibody [GT481] (GTX631108).Western blot analysis was performed using USP7 antibody [GT481] (GTX631108).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
