MA5-44335

antibody from Invitrogen Antibodies

Targeting: USP7 HAUSP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44335

Provider

Invitrogen Antibodies

Product name

USP7 Recombinant Rabbit Monoclonal Antibody (BLR072G)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR072G

Vial size

100 µL

Concentration

0.25 mg/mL

Storage

4°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP7 in whole cell lysate (50 µg) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:1,000 followed by HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of USP7 in FFPE section of OVCAR-4 cells. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of USP7 in Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using 20 µL/mg lysate. Also immunoprecipitated by rabbit anti-USP7 antibody (Product # A300-033A). For blotting immunoprecipitated USP7 monoclonal antibody was used at 1:1,000. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP7 in FFPE section of mouse renal cell carcinoma. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP7 in FFPE section of prostate carcinoma. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of USP7 in FFPE section of mouse renal cell carcinoma. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of USP7 in Jurkat cells. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) followed by DyLight® 488-conjugated goat anti-rabbit IgG secondary antibody. Isotype control (unshaded).