Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-44335 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP7 Recombinant Rabbit Monoclonal Antibody (BLR072G)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR072G
- Vial size
- 100 µL
- Concentration
- 0.25 mg/mL
- Storage
- 4°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP7 in whole cell lysate (50 µg) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:1,000 followed by HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of USP7 in FFPE section of OVCAR-4 cells. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of USP7 in Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using 20 µL/mg lysate. Also immunoprecipitated by rabbit anti-USP7 antibody (Product # A300-033A). For blotting immunoprecipitated USP7 monoclonal antibody was used at 1:1,000. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP7 in FFPE section of mouse renal cell carcinoma. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP7 in FFPE section of prostate carcinoma. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP7 in FFPE section of mouse renal cell carcinoma. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) using a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of USP7 in Jurkat cells. Samples were incubated with USP7 monoclonal antibody (Product # MA5-44335) followed by DyLight® 488-conjugated goat anti-rabbit IgG secondary antibody. Isotype control (unshaded).