Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP09237PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09237PU-N, RRID:AB_2036089
- Product name
- anti USP7 / HAUSP
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids near the amino terminus of Human HAUSP protein
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.98 mg/ml (by UV absorbance at 280 nm)
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using AP09237PU-N HAUSP antibody shows detection of HAUSP in various cell lysates at 130 kDa. A 1/500 dilution of the primary antibody was used for detection followed by a 1/5,000 dilution of HRP Goat anti-Rabbit IgG. Exposure time was 30 s.Lane 1: HeLa nuclear extractLane 2: HeLa Lane 3: A431 Lane 4: MCF7 Lane 5: 3T3 Lane 6-10: The antibody is blocked by pre-incubation with the immunizing peptide using the same lysates.
