PA1-39454

antibody from Invitrogen Antibodies

Targeting: CTNND1 CTNND, KIAA0384, p120, p120cas, p120ctn

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-39454

Provider

Invitrogen Antibodies

Product name

delta Catenin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast lobular carcinoma.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

1 mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells.

Jiang ZZ, Peng ZP, Liu XC, Guo HF, Zhou MM, Jiang D, Ning WR, Huang YF, Zheng L, Wu Y
Oncoimmunology 2022;11(1):2052418

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-delta Catenin Polyclonal Antibody (Product # PA5-32528) and ~108kDa band corresponding to CTNND1 was observed in all the cell lines tested except Jurkat and Daudi which are low expressing cell lines. An un-characterized band (*) was also observed at ~55kDa. Whole cell extracts (30 ug lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), A-431 (Lane 3), Jurkat (Lane 4), Daudi (Lane 5), NIH/3T3 (Lane 6), PC-3 (Lane 7) and HEK-293 (lane 8) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Catenin delta 1 (p120) using anti-Catenin delta 1 (p120) Polyclonal Antibody (Product # PA5-32528) in Breast Lobular Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. NETs induce the down-regulation of VE-cadherin on endothelial cells and correlate with tumor vasculature integrity . (a-c) CD15 + neutrophils were purified from peripheral blood of healthy donors. These cells were pre-treated with 30% non-tumor liver cell supernatants (N-SN) or primary HCC cancer cell supernatants (T-SN) for 12 hours, and then washed and cultured for another 4 hours to harvested NET (N-NET or T-NET, respectively). HUVEC cells were left untreated or treated with N-NET or T-NET for 12 hours. Their levels of p120 expression were quantified by western blotting. Levels of VE-cad expression in HUVEC were analyzed by both western blotting (a) and confocal microscopy (b) (n = 5). Red: VE-cad; Blue: DAPI. (d-f) CD15 + neutrophils were purified from peripheral blood of healthy donors and treated with 30% N-SN or T-SN for 12 hours (designated as N-Neu and T-Neu respectively) before being washed and co-cultured with HUVEC cells, in the presence or absence of DNase I. The wells were washed after 12 hours' co-culture to remove neutrophils, and levels of p120 expression in HUVEC cells were quantified by western blotting. Levels of VE-cad expression in HUVEC cells were analyzed by both western blotting (d) and confocal microscopy (e) (n = 5). Red: VE-cad; Blue: DAPI. (g, h) Paraffin-embedded HCC samples were stained with anti-human CD31 antibody (yellow), anti-human CD15 antibody (red), anti-human Cit-H3 antibody (green), anti-human VE-cad antibody (white), and DAP