orb1826269

antibody from Biorbyt

Targeting: NEUROG3 Atoh5, bHLHa7, Math4B, ngn3

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

orb1826269

Provider

Biorbyt

Product name

Neurogenin 3 Antibody / NGN3

Antibody type

Monoclonal

Description

This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

NGN3/7698

Vial size

100 ug

Storage

Aliquot the NEUROG3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.