AHO1552

antibody from Invitrogen Antibodies

Targeting: TGFBR1 ACVRLK4, ALK-5, ALK5, ESS1, MSSE, TBR-i, TBRI

Provider product page for AHO1552

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [3]
Comments [0]
Validations
Other assay [4]

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Product number: AHO1552 - Provider product page
Provider: Invitrogen Antibodies
Product name: TGFBR1 Monoclonal Antibody (234R32)
Antibody type: Monoclonal
Antigen: Recombinant protein fragment
Reactivity: Human, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 234R32
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20°C

TGFBR1 protein structure - AHO1552 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 OTUD4 regulates the ubiquitination of the TbetaR complex. ( A ) HEK293T cells were transfected with TbetaRI and/or FLAG-OTUD4. After 48 h cells were lysed and immunoprecipitated with anti- TbetaRI affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. ( B ) HEK293T cells were transfected with TbetaRII and FLAG-OTUD4. After 48 h cells were lysed and immunoprecipitated with anti- OTUD4 affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. ( C ) HEK293T cells were transfected with HA-ubiquitin, TbetaRI and either FLAG-OTUD4, or FLAG-OTUD4 DD. After 48 h cells were lysed and immunoprecipitated with anti- TbetaRI affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. ( D ) HEK293T cells were transfected with HA-ubiquitin, TbetaRII and either FLAG-OTUD4, or FLAG-OTUD4 DD. After 48 h cells were lysed and immunoprecipitated with anti-TbetaRII affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. ( E ) HEK293T cells were transfected with TbetaRI and either FLAG-OTUD4 or FLAG OTUD4 DD. Whole cell extracts were probed with indicated antibodies. beta-Actin is used as the loading control. ( F ) HEK293T cells were transfected with TbetaRII and either FLAG-OTUD4 or FLAG OTUD4 DD. Cells were stimulated where indicated with TGFbeta (100 pM) overnight before lysis. W

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 OTUD4 regulates TbetaRI presence at the plasma membrane. ( A ) HEK293T cells were transfected with TbetaRI and either FLAG-OTUD4 or FLAG-OTUD4 DD. Cells were stimulated with TGFbeta (100 pM) for the length of time indicated. Cell surface was labelled with biotin at 4 degrees for 40 min before lysis. Lysates were immunoprecipitated with anti- NeutrAvidin affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. beta-Actin is used as the loading control. ( B ) HEK293T cells were transfected with HA-ubiquitin, FLAG-TbetaRI C.A. and either FLAG-OTUD4 or FLAG-OTUD4 DD. Cells were incubated with MG132 (5 uM) overnight before lysis. After 48 h cells were lysed and immunoprecipitated with anti-TbetaRI affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. ( C ) HEK293T cells were transfected with FLAG-TbetaRI C.A. and either FLAG-OTUD4 or FLAG OTUD4 DD. Whole cell extracts were probed with indicated antibodies. beta-Actin is used as the loading control. ( D ) HEK293T cells were transfected with HA-ubiquitin, FLAG-TbetaRI C.A. and OTUD4 shRNA constructs B or C. Cells were incubated with MG132 (5 uM) overnight before lysis. After 72 h cells were lysed and immunoprecipitated with anti-TbetaRI affinity resin. Immunoprecipitated lysates and whole cell extracts were probed with the indicated antibodies. ( E ) HEK293T OTUD4 KD1 cells were transfected with FLAG-TbetaRI C.A. and

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 CD109 expression levels inversely correlate with TGF-beta signaling, EMT marker expression, cellular migration and invasion. ( A ) Isolation of CD109 H , CD109 M , and CD109 L subpopulations of A431 SCC cells by flow cytometry based on their CD109 expression levels. ( B ) Sorted cells were put in culture for three weeks and then re-analyzed by flow cytometry for CD109 expression, which showing that they maintain their respective CD109 expression profiles. ( C ) Representative image and ( D ) quantification of Western blot analysis of TGF-beta receptor I (ALK5) and P-Smad2 in CD109 H , CD109 M CD109 L cells, showing that CD109 expression levels are inversely correlated with TGF-beta signalling. ( E ) Representative image and ( F ) quantification of Western blot analysis for EMT markers in CD109 H , CD109 M , CD109 L cells, respectively. EMT markers expressions are inversely correlated with CD109 expression. ( G ) Representative image and ( H ) quantification of Immunofluorescence microscopy for CD109 (Green), Snail (Red) and DAPI (Blue) in CD109 H and CD109 L SCC cells, respectively, showing that CD109 H cells exhibited decreased Snail expression. ( I ) Representative images and ( J ) quantification of wound-healing assays on CD109 H , CD109 M , and CD109 L subpopulations as indicated, revealing that the levels of CD109 were inversely corelated with the migration of SCC cells. Cell migration was expressed as a percentage of the scratch area filled by migrating cells a

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 NaHS inhibited EMT in CS-exposed mouse lungs. After CS exposure for 12 weeks, mice were euthanized, and lung tissues were collected. (A) Western blot was used to analyze the protein levels of E-cadherin, CK18, Fibronectin, vimentin, FSP-1, alpha-SMA, Snail, MMP-2, MMP-9 and MMP-12 in the lung tissues. (B) Densitometric analysis of E-cadherin, CK18, Fibronectin, vimentin, FSP-1, alpha-SMA, Snail, MMP-2, MMP-9 and MMP-12 in the immunoblots using beta-actin as the internal reference. (C) The expression of E-cadherin and vimentin in the lungs were visualized using immunofluorescence double staining. (D, E) Western blot was used to analyze the protein levels of TGF-beta1, TGFbetaR1 and p-Smad3 in mouse lungs. Data are presented as mean +- SEM of 6 mice/group, *P < 0.05, **P < 0.01, significantly different from control group; # P < 0.05, ## P < 0.01, significantly different from CS + Saline group. Fig. 2