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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA1-32780 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-32780 detects Frizzled 6 from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 μg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ehrlichia chaffeensis TRP120 Is a Wnt Ligand Mimetic That Interacts with Wnt Receptors and Contains a Novel Repetitive Short Linear Motif That Activates Wnt Signaling.
Rogan MR, Patterson LL, Byerly CD, Luo T, Paessler S, Veljkovic V, Quade B, McBride JW
mSphere 2021 Apr 21;6(2)
mSphere 2021 Apr 21;6(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FZD6 in human liver tissue using FZD6 Polyclonal Antibody (Product # PA1-32780) at a dilution of 14 µg/mL. Antigen retrival : Heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human skeletal muscle tissue using (Product # PA1-32780) FZD6 Polyclonal Antibody. Antigen retrieval: Heat-induced antigen retrieval. Dilution: 14 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human liver tissue using (Product # PA1-32780) FZD6 Polyclonal Antibody. Antigen retrieval: Heat-induced antigen retrieval. Dilution: 14 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FZD6 in human skeletal muscle tissue using FZD6 Polyclonal Antibody (Product # PA1-32780) at a dilution of 14 µg/mL. Antigen retrival : Heat-induced antigen retrieval.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG 3 Small interfering RNA silencing of Wnt pathway receptor complex proteins significantly reduces E. chaffeensis infection. Small interfering RNA-transfected THP-1 cells were infected or mock infected with E. chaffeensis (MOI 100, 24 h posttransfection). (A) Infection level was quantified at 24 hpi by qPCR amplification of ehrlichial dsb for each siRNA treatment ( y axis). Scrambled siRNA (scrRNA) was transfected as a negative control. Values represent the mean plus or minus standard deviation. *, P < 0.05; ns, P > 0.05. Data are an average from at least three independent experiments. (B) Immunoblots depicting protein level of siRNA targets relative to scrRNA transfection from whole-cell lysates harvested 24 h posttransfection. Number above siRNA lane indicates percent knockdown of protein of interest relative to scrRNA transfection, normalized to GAPDH expression.
