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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF3149 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Frizzled-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Frizzled-6 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Frizzled-6 is observed, and less than 5% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, recombinant human (rh) Frizzled-5, rmFrizzled-7, rmFrizzled-8, and rhFrizzled-10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O60353- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
García-Castro B, Alvarez-Zavala M, Riveros-Magaña AR, Ortíz-Lazareno PC, Ratkovich-González S, Hernández-Flores G, Bravo-Cuellar A, Jave-Suarez LF, Aguilar-Lemarroy A
BMC cancer 2013 Nov 25;13:557
BMC cancer 2013 Nov 25;13:557
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Frizzled-6 in Human Kidney Cancer Tissue. Frizzled-6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
