Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA015313 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA015313, RRID:AB_1854343
- Product name
- Anti-NDRG4
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NDRG4, Gene description: NDRG family member 4, Alternative Gene Names: KIAA1180, SMAP-8, Validated applications: WB, IHC, Uniprot ID: Q9ULP0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients
Automated Learning of Subcellular Variation among Punctate Protein Patterns and a Generative Model of Their Relation to Microtubules
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
NDRG4 Is Required for Cell Cycle Progression and Survival in Glioblastoma Cells
Jandrey E, Moura R, Andrade L, Machado C, Campesato L, Leite K, Inoue L, Asprino P, da Silva A, de Barros A, Carvalho A, de Lima V, Carraro D, Brentani H, da Cunha I, Soares F, Parmigiani R, Chammas R, Camargo A, Costa É
npj Breast Cancer 2019;5(1)
npj Breast Cancer 2019;5(1)
Automated Learning of Subcellular Variation among Punctate Protein Patterns and a Generative Model of Their Relation to Microtubules
Tresch A, Johnson G, Li J, Shariff A, Rohde G, Murphy R
PLOS Computational Biology 2015;11(12):e1004614
PLOS Computational Biology 2015;11(12):e1004614
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
NDRG4 Is Required for Cell Cycle Progression and Survival in Glioblastoma Cells
Schilling S, Hjelmeland A, Radiloff D, Liu I, Wakeman T, Fielhauer J, Foster E, Lathia J, Rich J, Wang X, Datto M
Journal of Biological Chemistry 2009;284(37):25160-25169
Journal of Biological Chemistry 2009;284(37):25160-25169
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and NDRG4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402788).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-NDRG4 antibody. Corresponding NDRG4 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol