PA1-24628

antibody from Invitrogen Antibodies

Targeting: OPRM1 MOR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-24628

Provider

Invitrogen Antibodies

Product name

OPRM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-24628 is expected to cross react with bovine (93% conserved), guinea pig (100% conserved), Macaque monkey (100% conserved), and porcine (100% conserved) due to sequence homology.

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using OPRM1 Polyclonal Antibody (Product # PA1-24628) and ~53kDa band corresponding to OPRM1 was observed across cell lines and tissue extracts tested except in Mouse Kidney and Mouse Heart which are reported to be negative. An uncharacterized band (*) at ~110, ~15 kDa was observed in the Rat Brain and Mouse Lungs respectively. Whole cell extracts (30 µg lysate) of (Fig. a) SH-SY5Y (Lane 1), SK-N-AS (Lane 2), IMR-32 (Lane 3), U-2 OS (Lane 4), MCF7 (Lane 5), Neuro-2a (Lane 6), A549 (Lane 7), tissue extracts (30ug lysate) of Mouse Testis (Lane 8), Mouse Brain (Lane 9), Rat Brain (Lane 10) and Mouse Lungs (Lane 11); (Fig. b) tissue extracts (30ug lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Testis (Lane 3), Rat Testis (Lane 4), Mouse Lungs (Lane 5), Mouse Kidney (Lane 6) and Mouse Heart (Lane 7) were electrophoresed using NuPAGE® 10% Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mu Opioid Receptor using a mu Opioid Receptor polyclonal antibody (Product # PA1-24628). The image shows the presence of a subpopulation of spinal lamina II neurons that contained both µ opioid receptor (green) and TRPV1 (red) immunoreactivity in a vehicle (A)- and a RTX (B)-treated rats. Co-localization of the µ opioid receptor and TRPV1 immunoreactivity is indicated in yellow when 2 images are digitally merged. All images are single confocal optical sections. Scale bar: 20 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mu Opioid Receptor in rat DRG using a mu Opioid Receptor polyclonal antibody (Product # PA1-24628) at a dilution of 1:100. Cells were incubated at 4ºC overnight and detected using an anti-Rabbit Rhodamine Red secondary antibody at a dilution of 1:200, incubated at room temperature for 1 hour.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of rat DRG tissue using OPRM1 Polyclonal Antibody (Product # PA1-24628). Dilution: 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of striatum using (Product # PA1-24628) OPRM1 Polyclonal Antibody.