600-101-391

antibody from Rockland Immunochemicals, Inc.

Targeting: RAD23B HHR23B, HR23B, P58

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-101-391

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-101-391, RRID:AB_2253497

Product name

Anti-HR23B (GOAT) Antibody - 600-101-391

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate.  Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown).   Approximately 33 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] (605-432-013) for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

SDS PAGE