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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB4729 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal C5L2/GPR77 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody stains mouse C5L2 transfectants but not irrelevant transfectants.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references T Cell Expression of C5a Receptor 2 Augments Murine Regulatory T Cell (TREG) Generation and TREG-Dependent Cardiac Allograft Survival.
Monitoring C5aR2 Expression Using a Floxed tdTomato-C5aR2 Knock-In Mouse.
A Verghese D, Demir M, Chun N, Fribourg M, Cravedi P, Llaudo I, Woodruff TM, Yadav P, Lira SA, Medof ME, Heeger PS
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 15;200(6):2186-2198
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 15;200(6):2186-2198
Monitoring C5aR2 Expression Using a Floxed tdTomato-C5aR2 Knock-In Mouse.
Karsten CM, Wiese AV, Mey F, Figge J, Woodruff TM, Reuter T, Scurtu O, Kordowski A, Almeida LN, Briukhovetska D, Quell KM, Sun J, Ender F, Schmudde I, Vollbrandt T, Laumonnier Y, Köhl J
Journal of immunology (Baltimore, Md. : 1950) 2017 Nov 1;199(9):3234-3248
Journal of immunology (Baltimore, Md. : 1950) 2017 Nov 1;199(9):3234-3248
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Monoclonal Antibody (Catalog # MAB4729, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
