703357

antibody from Invitrogen Antibodies

Targeting: GABBR2 GABABR2, GPR51, GPRC3B, HG20

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703357

Provider

Invitrogen Antibodies

Product name

GABBR2 Recombinant Rabbit Monoclonal Antibody (19H3L12)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

19H3L12

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HEK-293 cells were stably transfected with human GABBR2. After 24hr of transfection the cells were fixed and permeabilized for detection of GABBR2 using Anti-GABBR2 Recombinant Rabbit Monoclonal Antibody (Product # 703357, 1 µg/mL, 1:500 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Panel a) shows representative cells that were stained for detection of membrane localization of the GABBR2 on the transfected cells. The images were captured at 40X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of Rat and Human Brain cortex were dewaxed, microwaved in citric acid and incubated with Anti-GABBR2 Recombinant Rabbit Monoclonal Antibody (Product # 703357, 1 µg/mL, 1:500 dilution). Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were further developed in AEC and lightly counterstained with hematoxylin. Panel a & b) shows the specific localization of GABBR2 in neuronal stomata and dendrites in both Rat and Human brain cortex respectively. Panel c) shows the no primary antibody control.