PA5-47869

antibody from Invitrogen Antibodies

Targeting: CELSR2 ADGRC2, CDHF10, EGFL2, Flamingo1, KIAA0279, MEGF3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47869

Provider

Invitrogen Antibodies

Product name

CELSR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR2. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for CELSR2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CELSR2 in HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR2. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for CELSR2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CELSR2 in immersion fixed paraffin-embedded sections of human breast. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to ductal epithelium.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47869, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47869, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CELSR2 in SH‚SY5Y human neuroblastoma cell line. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CELSR2 in bEnd.3 mouse endothelioma cell line. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.