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antibody from Invitrogen Antibodies
Targeting: CELSR2
ADGRC2, CDHF10, EGFL2, Flamingo1, KIAA0279, MEGF3
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- PA5-47869 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CELSR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed. Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CELSR2 in immersion fixed paraffin-embedded sections of human breast. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to ductal epithelium.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47869, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47869, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CELSR2 in SH‚SY5Y human neuroblastoma cell line. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CELSR2 in bEnd.3 mouse endothelioma cell line. Samples were incubated in CELSR2 polyclonal antibody (Product # PA5-47869) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
