Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-22424 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal mGluR5/1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 130kDa (both mGluR1 and mGluR5).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: metabotropic Glutamate Receptor 5/1 Antibody (S75-3) [NBP2-22424] - analysis of Rat Brain Membrane showing detection of 130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33 . Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 130 kDa.