PA5-36866

antibody from Invitrogen Antibodies

Targeting: PAK3 bPAK, hPAK3, MRX30, MRX47

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36866

Provider

Invitrogen Antibodies

Product name

Phospho-PAK3 (Ser154) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 65 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK3 (Ser154) in Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 2: sp2/0 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 mins). Samples were incubated with Phospho-PAK3 (Ser154) polyclonal antibody (Product # PA5-36866) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK3 pSer154 using Phospho-PAK3 pSer154 polyclonal antibody (Product # PA5-36866) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: sp2/0 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK3 (Ser154) in Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 2: sp2/0 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 mins). Samples were incubated with Phospho-PAK3 (Ser154) polyclonal antibody (Product # PA5-36866) at a dilution of 1:500.