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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C45675 - Provider product page
- Provider
- LSBio
- Product name
- Anti-EP300 / p300 Antibody LS-C45675
- Antibody type
- Polyclonal
- Description
- Purified IgG
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µg
- Storage
- Store at +4°C. DO NOT FREEZE.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Detection of p300 in varying amounts of whole cell lysates from HeLa cells, as indicated in A. with Rabbit anti-Human p300 (LS-C45675). Immunoprecipitation of a nuclear extract (NE) or a cytosolic (S100) extract from HeLa cells with 20 ug/10 mg lysate with LS-C45675 followed by resolving of immunoprecipitate by SDS page and Coomassie staining. Identity of p300 band was confirmed by mass spectroscopy.
