Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9178 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-KAT3B/p300/EP300 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for p300/EP300 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. p300/EP300 information: Molecular Weight: 264161 MW; Subcellular Localization: Cytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast.
MAFG-AS1/MAFG positive feedback loop contributes to cisplatin resistance in bladder urothelial carcinoma through antagonistic ferroptosis.
Xu P, Yi Y, Xiong L, Luo Y, Xie C, Luo D, Zeng Z, Liu A
International journal of radiation oncology, biology, physics 2024 Jan 1;118(1):203-217
International journal of radiation oncology, biology, physics 2024 Jan 1;118(1):203-217
MAFG-AS1/MAFG positive feedback loop contributes to cisplatin resistance in bladder urothelial carcinoma through antagonistic ferroptosis.
Xiang L, Zeng Q, Liu J, Xiao M, He D, Zhang Q, Xie D, Deng M, Zhu Y, Liu Y, Bo H, Liu X, Zhou M, Xiong W, Zhou Y, Zhou J, Li X, Cao K
Science bulletin 2021 Sep 15;66(17):1773-1788
Science bulletin 2021 Sep 15;66(17):1773-1788
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of KAT3B/p300 using anti-KAT3B/p300 antibody (PB9178). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human kat3b/p300 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/p300 antigen affinity purified polyclonal antibody (Catalog # PB9178) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/p300 at approximately 50KD. The expected band size for KAT3B/p300 is at 50KD.
- Additional image