PAB1652

antibody from Abnova Corporation

Targeting: EP300 KAT3B, p300

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB1652

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB1652, RRID:AB_1238058

Product name

EP300 (phospho S1834) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of EP300.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

EP2 and EP4 receptors regulate aromatase expression in human adipocytes and breast cancer cells. Evidence of a BRCA1 and p300 exchange.

Subbaramaiah K, Hudis C, Chang SH, Hla T, Dannenberg AJ
The Journal of biological chemistry 2008 Feb 8;283(6):3433-44

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Skp2 suppresses p53-dependent apoptosis by inhibiting p300.

Kitagawa M, Lee SH, McCormick F
Molecular cell 2008 Feb 1;29(2):217-31

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human spleenreacted with EP300 (phospho S1834) polyclonal antibody (Cat # PAB1652), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of EP300 (phospho S1834) polyclonal antibody (Cat # PAB1652) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)