Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PAB1652 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1652, RRID:AB_1238058
- Product name
- EP300 (phospho S1834) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of EP300.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references EP2 and EP4 receptors regulate aromatase expression in human adipocytes and breast cancer cells. Evidence of a BRCA1 and p300 exchange.
Skp2 suppresses p53-dependent apoptosis by inhibiting p300.
Subbaramaiah K, Hudis C, Chang SH, Hla T, Dannenberg AJ
The Journal of biological chemistry 2008 Feb 8;283(6):3433-44
The Journal of biological chemistry 2008 Feb 8;283(6):3433-44
Skp2 suppresses p53-dependent apoptosis by inhibiting p300.
Kitagawa M, Lee SH, McCormick F
Molecular cell 2008 Feb 1;29(2):217-31
Molecular cell 2008 Feb 1;29(2):217-31
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human spleenreacted with EP300 (phospho S1834) polyclonal antibody (Cat # PAB1652), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of EP300 (phospho S1834) polyclonal antibody (Cat # PAB1652) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)