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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358187 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-E1A Binding Protein P300 (EP300) (pSer89) antibody
- Antibody type
- Polyclonal
- Description
- Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer89
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53.
The N-terminal interferon-binding domain (IBiD) homology domain of p300 binds to peptides with homology to the p53 transactivation domain.
Role of Akt/protein kinase B in the activity of transcriptional coactivator p300.
Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway.
Transforming growth factor-beta stimulates p300-dependent RUNX3 acetylation, which inhibits ubiquitination-mediated degradation.
Dornan D, Eckert M, Wallace M, Shimizu H, Ramsay E, Hupp TR, Ball KL
Molecular and cellular biology 2004 Nov;24(22):10083-98
Molecular and cellular biology 2004 Nov;24(22):10083-98
The N-terminal interferon-binding domain (IBiD) homology domain of p300 binds to peptides with homology to the p53 transactivation domain.
Finlan L, Hupp TR
The Journal of biological chemistry 2004 Nov 19;279(47):49395-405
The Journal of biological chemistry 2004 Nov 19;279(47):49395-405
Role of Akt/protein kinase B in the activity of transcriptional coactivator p300.
Chen J, Halappanavar SS, St-Germain JR, Tsang BK, Li Q
Cellular and molecular life sciences : CMLS 2004 Jul;61(13):1675-83
Cellular and molecular life sciences : CMLS 2004 Jul;61(13):1675-83
Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway.
Kung AL, Zabludoff SD, France DS, Freedman SJ, Tanner EA, Vieira A, Cornell-Kennon S, Lee J, Wang B, Wang J, Memmert K, Naegeli HU, Petersen F, Eck MJ, Bair KW, Wood AW, Livingston DM
Cancer cell 2004 Jul;6(1):33-43
Cancer cell 2004 Jul;6(1):33-43
Transforming growth factor-beta stimulates p300-dependent RUNX3 acetylation, which inhibits ubiquitination-mediated degradation.
Jin YH, Jeon EJ, Li QL, Lee YH, Choi JK, Kim WJ, Lee KY, Bae SC
The Journal of biological chemistry 2004 Jul 9;279(28):29409-17
The Journal of biological chemistry 2004 Jul 9;279(28):29409-17
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