NB100-616

antibody from Novus Biologicals

Targeting: EP300 KAT3B, p300

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-616

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-616, RRID:AB_10002598

Product name

Mouse Monoclonal KAT3B/p300 Antibody

Antibody type

Monoclonal

Description

Protein G purified. This is specific for p300 protein. This recognizes residues 1921-2023.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

MLL4 Is Required to Maintain Broad H3K4me3 Peaks and Super-Enhancers at Tumor Suppressor Genes.

Dhar SS, Zhao D, Lin T, Gu B, Pal K, Wu SJ, Alam H, Lv J, Yun K, Gopalakrishnan V, Flores ER, Northcott PA, Rajaram V, Li W, Shilatifard A, Sillitoe RV, Chen K, Lee MG
Molecular cell 2018 Jun 7;70(5):825-841.e6

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KLF4 mediates the link between TGF-β1-induced gene transcription and H3 acetylation in vascular smooth muscle cells.

He M, Zheng B, Zhang Y, Zhang XH, Wang C, Yang Z, Sun Y, Wu XL, Wen JK
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Sep;29(9):4059-70

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Structure of a biologically active estrogen receptor-coactivator complex on DNA.

Yi P, Wang Z, Feng Q, Pintilie GD, Foulds CE, Lanz RB, Ludtke SJ, Schmid MF, Chiu W, O'Malley BW
Molecular cell 2015 Mar 19;57(6):1047-1058

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Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter.

Byun SW, Chang YJ, Chung IS, Moss SF, Kim SS
Cancer letters 2012 Dec 29;326(1):96-104

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Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.

Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM
Genes & development 1999 Jan 1;13(1):64-75

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Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.

Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM
Genes & development 1999 Jan 1;13(1):64-75

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Polyomavirus large T antigen binds the transcriptional coactivator protein p300.

Nemethova M, Wintersberger E
Journal of virology 1999 Feb;73(2):1734-9

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Polyomavirus large T antigen binds the transcriptional coactivator protein p300.

Nemethova M, Wintersberger E
Journal of virology 1999 Feb;73(2):1734-9

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Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.

Eckner R, Yao TP, Oldread E, Livingston DM
Genes & development 1996 Oct 1;10(19):2478-90

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Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.

Eckner R, Yao TP, Oldread E, Livingston DM
Genes & development 1996 Oct 1;10(19):2478-90

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Association of p300 and CBP with simian virus 40 large T antigen.

Eckner R, Ludlow JW, Lill NL, Oldread E, Arany Z, Modjtahedi N, DeCaprio JA, Livingston DM, Morgan JA
Molecular and cellular biology 1996 Jul;16(7):3454-64

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Association of p300 and CBP with simian virus 40 large T antigen.

Eckner R, Ludlow JW, Lill NL, Oldread E, Arany Z, Modjtahedi N, DeCaprio JA, Livingston DM, Morgan JA
Molecular and cellular biology 1996 Jul;16(7):3454-64

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Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.

Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM
Genes & development 1994 Apr 15;8(8):869-84

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Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.

Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM
Genes & development 1994 Apr 15;8(8):869-84

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW105) antibody NB100-616 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the PE format of this antibody.